Biospective has its own in-house, state-of-the-art histopathology laboratory to provide a full range of pathology services to its clients. We have expertise performing studies using a broad spectrum of tissues (such as brain, lung, tumor) and stains. One of our particular strengths is quantitative image analysis of digitized histology/IHC slides using our unique PERMITS™ software. All studies are performed with strict attention to quality control.


Complete Histology Services

  • Perfusion fixation and tissue dissection
  • Tissue embedding & processing
  • FFPE & frozen tissue sectioning
  • Histological & IHC staining
  • Whole slide digitization
  • Zeiss MIRAX Scan 150


Quantitative Image Analysis (PERMITS™)

  • Brain

  • Beta-amyloid burden
  • Myelin quantification
  • Neuronal density
  • Neuroinflammation
  • Vascular density
  • MRI-IHC co-registration
  • Lung

  • Goblet cell hyperplasia
  • Eosinophil quantification
  • Customized applications


Commonly Requested Stains

  • Histology

  • H&E
  • Cresyl violet
  • Luxol Fast Blue (LFB)
  • Perl’s Iron/Prussian Blue
  • Masson’s Trichrome
  • Periodic acid-Schiff (PAS)
  • Acid Blue 129
  • Immunohistochemistry

  • Beta-amyloid
  • NeuN (neurons)
  • GFAP (astrocytes)
  • IBA-1 (macrophages/microglia)
  • Alpha-synuclein
  • Neurofilament (axons)
  • Synaptophysin (synapses)
  • MBP, PLP (myelin)
  • Collagen IV (vessels)
  • Tau
  • APP/SMI-32 (axonal damage)
  • IgG/Fibrinogen
    (vasogenic edema)
  • Inflammatory cell markers

Download our Histopathology Services brochure

Posters & Publications

Zehntner SP, Grand’Maison M, Wood A, Zijdenbos AP, Steff A-M, Bedell BJ. Automated quantitative image analysis of goblet cell hyperplasia in a mouse model of RSV infection. RSV Vaccines for the World Congress, 2013, Porto, Portugal.
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Ho M-K, Zijdenbos AP, Zehntner SP, Hébert F, DeDuck K, Grand’Maison M, Bedell BJ. Cortical laminar analysis of β-amyloid deposition, astrogliosis, and neuronal density in a mouse model of Alzheimer’s disease. Alzheimer Association International Conference, 2013, Boston, MA, USA.
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Zehntner SP, Chakravarty MM, Bolovan RJ, Chan C, Bedell BJ. Synergistic tissue counterstaining and image segmentation techniques for accurate, quantitative immunohistochemistry. J Histochem Cytochem 2008;56(10):873-880.
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Customized Staining

Biospective prides itself on developing customized staining protocols for your specific needs. Please contact us to discuss your projects.

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